Fig. 5: Quantitative detection of RNA by SCas12a assay.
From: Split crRNA with CRISPR-Cas12a enabling highly sensitive and multiplexed detection of RNA and DNA
a Selective detection of the target miR-21 (10 pM) in the different types of miRNAs (1 nM), including miR-17, miR-31 and miR-let-7a. b Multiplexed detection of miR-17 and miR-31 in the same reaction. c Heatmap of the estimated concentrations of target miR-21 using the SCas12a assay or conventional RT-qPCR assay. d Schematic of an HIV RNA target and three ssDNA activators targeting it at different positions. The predicted secondary structure of this HIV RNA was determined using NUPACK software. e Comparison among the head, mid, tail and pooled HIV targeting ssDNA activators. f Schematic of the detection of mature miRNA and pre-miRNA using SCas12a or Cas13a based fluorescence assay. g Comparison of fluorescence intensity changes between pre miR-21 and mature miR-21 measured using Cas13a, SCas12a or Asymmetric CRISPR assay. The concentrations of pre-miR-21 and mature miR-21 were both 10 nM in each reaction. For a, b, e, and g, all the experiments were conducted in triplicate and error bars represent mean value +/− SD (n = 3). Source data are provided as a Source Data file.
