Fig. 7: Specificity of the SCas12a assay towards single point mutations in DNA target.
From: Split crRNA with CRISPR-Cas12a enabling highly sensitive and multiplexed detection of RNA and DNA
a Split RNA (SCas12a) and crRNA (WT Cas12a) activators were designed with point mutations across the length of the pairing region in a HPV16-derived DNA target. The mutation location is identified by ‘M’ following the nucleotide number where the base has been changed to its complementary deoxynucleotide (3’ to 5’ direction). b Comparison of fluorescence fold changes for the trans-cleavage between wild-type Cas12a assay and the SCas12a assay. All fluorescence values were normalized to those of the WT crRNA activator. Statistical analysis for n = 3 biologically independent replicates comparing the normalized fold change for the WT Cas12a assay vs. SCas12a assay. Statistical analysis was conducted using a two-tailed t-test. Statistical significance was determined as follows: ns (not significant) for p > 0.05, * for p ≤ 0.05, ** for p ≤ 0.01, *** for p ≤ 0.001, and **** for p ≤ 0.0001. Eerror bars represent mean value +/− SD (n = 3). Source data are provided as a Source Data file.
