Table 1 Semen analysis in Cep112+/+ and Cep112−/− male mice by CASA

From: CEP112 coordinates translational regulation of essential fertility genes during spermiogenesis through phase separation in humans and mice

 

Adult male mice

P value

Cep112+/+

Cep112−/−

 

Semen parameters

 Sperm concentration (106/ml)a

32.17 ± 4.42

6.14 ± 1.69

0.0007

 Motility (%)b

74.23 ± 3.50

4.50 ± 0.54

<0.0001

 Progressive motility (%)b

71.51 ± 4.13

2.01 ± 0.94

<0.0001

Sperm locomotion parameters

 Curvilinear velocity (VCL) (μm/s)b

91.89 ± 7.59

7.80 ± 2.23

<0.0001

 Straight-line velocity (VSL) (μm/s)b

41.86 ± 6.26

1.16 ± 0.23

0.0004

 Average path velocity (VAP) (μm/s)b

58.05 ± 2.18

3.00 ± 0.51

<0.0001

 Amplitude of lateral head displacement (ALH) (μm)b

1.28 ± 0.22

0.18 ± 0.04

0.0010

 Linearity (LIN)b

0.45 ± 0.03

0.15 ± 0.02

0.0001

 Wobble (WOB, =VAP/VCL)b

0.63 ± 0.03

0.40 ± 0.07

0.0064

 Straightness (STR, =VSL/VAP)b

0.72 ± 0.08

0.38 ± 0.02

0.0020

 Beat-cross frequency (BCF) (Hz)b

7.13 ± 0.67

0.43 ± 0.06

<0.0001

  1. aEpididymides and vas deferens.
  2. bn  =  3 biologically independent Cep112+/+ mice or Cep112−/− mice. Statistical analysis was performed using a two-sided Student’s t test. P-values < 0.05 were considered statistically significant.