Fig. 1: Transcriptomic analysis of the M2IL4 and M2GC populations.
a Transcriptomic changes in M0 macrophages polarized with IL4 or GCs corticosterone (Cort) or dexamethasone (Dex) for 24 h were determined by RNAseq (n = 3 biological replicates [mice]). Venn diagrams show DEGs in M2IL4 and M2GC relative to M0 (FC ≥ 2; FDR < 0.05); 92 of 133 genes are regulated in both M2IL4 and M2Dex in the same direction. b Volcano plots show DEGs in M2IL4 (left) and M2Dex (right) relative to M0 (LogFC ≥ 1; FDR < 0.05). Selected upregulated genes are highlighted in orange (M2IL4) and green (M2Dex). Downregulated genes are highlighted in red in both populations. Examples of the M2IL4:M2Dex shared DEGs are underlined. c RT-qPCR validation of genes upregulated selectively in M2IL4 (top), M2GC (middle), or both (bottom). One-tailed unpaired t test; p-values are shown; ns, non-significant (p ≥ 0.05). n = 4 biological replicates (mice), error bars are SEM. d Differentially regulated pathways (unadjusted p < 0.01; see ref. 71. equations 14-15) identified using QuSAGE and MsigDB canonical pathways (c2.cp.v7.3, Broad Institute) are shown for M2IL4 (top) and M2Dex (bottom). Underlined are the shared pathways between M2IL4 (upregulated, orange; downregulated, red) and M2Dex (upregulated, green; downregulated, red). Circle size is proportional to the number of genes in the pathway, and color signifies the p-value. e Genes from indicated pathways induced or repressed in M2IL4, M2Dex, or both are plotted as LogFC ± SD. Source data are provided as a Source data file.
