Fig. 8: Enzyme engineering. | Nature Communications

Fig. 8: Enzyme engineering.

From: Elucidation of the noncovalent interactions driving enzyme activity guides branching enzyme engineering for α-glucan modification

Fig. 8: Enzyme engineering.

a Top ten variants amid the forty-seven ones. The WT RpBE is regarded as possessing 100% enzyme activity. b Mutation sites (shown in sphere) around the CceBE1 G7-pose i. The catalytic triad is shown in a licorice representation, and colored in yellow (carbon atoms). c Distribution of the glycan chain length. The data without enzyme catalysis are not shown for clarity. The two-tailed p values were obtained by the unpaired t test analysis using Graphpad Prism 8. No adjustment was made for multiple comparisons. The bars and the error bars are the average and standard deviation of triplicates (n = 3 independent experiments), respectively. Source data for a, c are provided as a Source Data file.

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