Fig. 1: Glyco-lipidomic profiling of region-resolved adult mouse brain.
From: Deciphering mouse brain spatial diversity via glyco-lipidomic mapping
a Brain tissue preparation. Multiple regions of the mouse brain were dissected, and following tissue homogenization, gangliosides were enriched using a modified Folch method and C18 Solid Phase Extraction (SPE). CB cerebellum, CC cerebral cortex, DC diencephalon, Hip hippocampus, MB midbrain, OB olfactory bulb, PFC prefrontal cortex, P-M pons-medulla, ST striatum. b LC–MS/MS Data Acquisition. Ganglioside isomers were separated, and the obtained qualitative and quantitative data were subjected to a bioinformatic approach for comparative regional profiling. c Biological interpretation. Integration of biosynthesis pathways and a glyco-centric approach was performed to garner the functional roles of gangliosides in neurological processes. Some elements in this figure were created with BioRender.com, released under a Creative Commons Attribution 4.0 International license, https://creativecommons.org/licenses/by/4.0/.
