Fig. 3: NAT10 deficiency alleviates T cell-mediated colitis and experimental autoimmune encephalomyelitis in mice.

a, b Rag1^–/– mice were intravenously (i.v.) injected the CD4⁺CD25^-CD45RB^hi naïve T cells isolated from NAT10^WT (n = 5) or NAT10^cKO mice (n = 5). The body weight (a) and survival rate (b) of receipt mice were monitored every 2 days. p value and significance were determined by two-tailed Log-rank test use default parameters. c Representative H&E staining slides of sections from proximal colon of NAT10^WT (n = 3) or NAT10^cKO mice (n = 5). The top scale bar indicates 200 μm, the bottom one indicates 50 μm. d FACS analysis of NAT10^WT (n = 3) or NAT10^cKO mice (n = 5) derived CD4⁺ T cells infiltration in iLN, spleen, mesenteric lymph nodes (mLN) and lamina propria (LP) of RAG1^-/- recipients. e LP infiltrated immunocytes and splenocytes isolated from recipients transferred naïve T cells from NAT10^WT (n = 3) or NAT10^cKO mice (n = 5) were stimulated with PMA plus ionomycin, and IFN-γ production were analyzed by FACS. f NAT10^WT (n = 11) and NAT10^cKO (n = 7) mice were induced experimental autoimmune encephalomyelitis (EAE) model, the clinical scores were monitored from 5th day after immunization. g On the 14th day after immunization, the brains and spinal cords of the immunized mice were collected and dissociated, and immunocytes infiltration was analyzed by FACS (n = 3). The bar graphs were presented the percentage and absolute cell number of distinct immune cells subsets. h Representative H&E staining of spinal cord sections from mice immunized for 14 days. i–j Splenocytes of immunized mice were re-stimulated with 10 µg/mL MOG33-35 peptide and subjected to CCK8-based proliferation assay and ELISA-based cytokines production assay (n = 3). Source data are provided as a Source Data file. Data were represented as mean ± S.D. Three independent experiments at least were performed in data represented above. Statistical significance was determined by two-sided multiple t test (a, f), one-way ANOVA with multiple comparisons (d, h, i, j) and two-tailed t test (c, e). *P < 0.05, **P < 0.01, ***P < 0.001.