Fig. 5: NAT10 deficiency in T cells causes significantly reduce in various metabolic processing with a slight impairment of the TCR signal. | Nature Communications

Fig. 5: NAT10 deficiency in T cells causes significantly reduce in various metabolic processing with a slight impairment of the TCR signal.

From: N-acetyltransferase 10 is implicated in the pathogenesis of cycling T cell-mediated autoimmune and inflammatory disorders in mice

Fig. 5

a–d Naïve CD4+ T cells isolated from NAT10WT and NAT10cKO mice were stimulated with anti-CD3 plus anti-CD28 for indicated time. The activation of MAPKs (a), NF-κB (b), NFAT (c) and AKT (d) signaling pathway were measured by IB. Extracellular acidification rate (ECAR) (e) and oxygen consumption rate (OCR) (f) of WT and NAT10-deficient naïve CD4+ T cells stimulated with anti-CD3 plus anti-CD28 for 18 h were measured by Agilent Seahorse XF Analyzers (n = 3). Heatmap of different expression level of glycolysis (g) and fatty acid metabolism (h) associated genes between WT and NAT10-deficient naïve T cells stimulated with anti-CD3 plus anti-CD28 for 24 h. Source data are provided as a Source Data file. Data were represented as mean ± S.D. Three independent experiments at least were performed in data represented above.

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