Fig. 2: DM1 induces global transcriptomic alterations in hepatocytes.
a RT-PCR splicing analysis of select MBNL1 targets (n = 5 biological replicates). Bands indicate exon presence or absence, with (+) for inclusion and (-) for exclusion. Targets are listed to the left of the image, with the percentage “spliced in” (PSI) below. b Overlap of alternative splicing, alternative polyadenylation (APA), and expression changes upon DM1 induction in hepatocytes. c Volcano Plot illustrating mRNA abundance changes from RNA-seq as identified from DESeq2. d Violin plots displaying inclusion levels of alternative splicing events from RNA-seq data. MXE mutually exclusive, A3’SS/A5’SS alternative 3’/5’ splice sites, ASE alternative cassette exon, RI retained intron. e Change in PSI determined by RT-PCR (y-axis) vs. RNA Seq analysis (x-axis) for 30 events. f Gene Tracks of representative genes showing alternative exon inclusion in DM1 liver, Mbnl1∆E3/∆E3 (KO), or wild-type animals. g Pie chart – comparison of alternatively spliced genes regulated by DM1, MBNL1, or maturation in the liver. h Pie chart – comparison of differentially expressed genes (DEG) regulated by DM1, MBNL1, or maturation. i GO Diagram - mRNA Processing: Selected processes related to genes with alternative mRNA processing events in DM1 liver. j GO Diagram - Differential Expression: Processes related to genes undergoing differential expression in DM1 liver. b–j For the RNA-seq datasets, n = 3 for ApoE-rtTA control, n = 3 for DM1 liver mice, n = 2 for Mbnl1 WT, and n = 2 for Mbnl1 KO mice. Source data are provided as a Source Data file.
