Fig. 5: Binding mode of i6A.
From: Structural insights into the agonist selectivity of the adenosine A3 receptor
a Superimposition of adenosine- and i6A-bound A3R. b Binding mode of i6A. Black dashed lines indicate hydrogen bonds. c Close-up view of the hydrophobic pocket of the i6A-bound structure. d–f Relative RAi values of adenosine and i6A for human A3R mutants, as determined by the TGFα-shedding assay. The LogRAi cutoff value was set to −2. Mutations that reduce (d) and enlarge (e) the size of the hydrophobic pocket. f Mutations that replace the original residue to a hydrophilic residue. Data are presented as mean values ± SEM from at least three independent experiments performed in triplicate (the n values are represented by circles). The values for adenosine used references in (d–f) are the same as those for adenosine in Fig. 4e–g. Source data are provided as a Source Data file.
