Fig. 5: Gnrh1 and Oxt gene methylation and transcription in middle age or 5xFAD model. | Nature Communications

Fig. 5: Gnrh1 and Oxt gene methylation and transcription in middle age or 5xFAD model.

From: Targeting the hypothalamus for modeling age-related DNA methylation and developing OXT-GnRH combinational therapy against Alzheimer’s disease-like pathologies in male mouse model

Fig. 5

A, B Differentially methylated cytosines (DMCs) were analyzed in BOCS metadata covering Oxt gene (A) and Gnrh1 gene (B) for the hypothalamic samples of mice between 2 months and 12 months of age. The graphs present the DMCs with at least 1% methylation difference, with genomic positions of cytosines labeled on the Y axis. C, D The mRNA levels of Oxt and Gnrh1 measured in hypothalamic tissues from male C57BL/6 mice under middle age (15 months) vs. young age (2 months) (C) and from male 5xFAD mice vs. male littermate wildtype (WT) controls of 8 months of age (D). Statistics: For panels A and B, analyses were performed using logistic regression with the MethylKit package, with q-values < 0.05 indicating significance (two-sided test), and n = 3 mice per group. Data in panels C and D are presented as mean values ± SEM. au arbitrary unit. Statistical significance was assessed using two-tailed unpaired t-test, with p-values reported, and n = 5 mice per group. Source data are provided as a Source Data file.

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