Fig. 5: SoL A primarily suppresses LPS-induced TLR4 activation. | Nature Communications

Fig. 5: SoL A primarily suppresses LPS-induced TLR4 activation.

From: Biosynthetic enzyme analysis identifies a protective role for TLR4-acting gut microbial sulfonolipids in inflammatory bowel disease

Fig. 5: SoL A primarily suppresses LPS-induced TLR4 activation.

Mouse peritoneal macrophages were treated with SoL A (10 μM), LPS (100 ng/mL), and Pam3CSK4 (500 ng/mL), either alone or in combination for 6 h. RT-qPCR analysis revealed that SoL A induces a mild pro-inflammatory effect compared to control but significantly suppresses LPS-induced cytokine expression levels and only partially suppresses Pam3CSK4-induced cytokine expression. Bars represent mean ± standard error. Experiments were independently repeated three times. For each treatment, n = 3 individual wells. Significance was determined using two-way ANOVA. For all p values: *p < 0.05, ****p < 0.0001. Source data are provided in the Source Data file.

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