Fig. 7: Regulatory balance between HuSH and HuSH2 in K-562 cells. | Nature Communications

Fig. 7: Regulatory balance between HuSH and HuSH2 in K-562 cells.

From: Interplay between Two Paralogous Human Silencing Hub (HuSH) Complexes in Regulating LINE-1 Element Silencing

Fig. 7

HuSH and HuSH2 exist in a critical balance, each occupying distinct loci within the genome, as demonstrated by MPP8 ChIP-seq analysis (right panel). In the absence of either TASOR or TASOR2, the targeted complex fails to form, resulting in the inability of MPP8 to localize to the chromatin targeted by the missing complex. Consequently, an excess of MPP8 is available to stabilize and enhance the still intact complex. This redistribution of MPP8 from HuSH or HuSH2 sites leads to alterations in the silencing of LINE-1 elements, immune response-related genes (ISGs), and KRAB-ZNFs (ZNFs) in K-562 cells. The shift in MPP8 localization thereby significantly impacts the transcriptional regulation of these genomic elements, highlighting the delicate interplay between HuSH and HuSH2 in maintaining genomic stability and gene expression homeostasis in K-562 cells. Created in BioRender. Jensvold, Z. (2024) BioRender.com/z18k565.

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