Fig. 1: Impact of 8-oxodGuo location on specific binding by hOGG1K249Q.
From: Structural basis for human OGG1 processing 8-oxodGuo within nucleosome core particles
a X-ray crystal structure of a NCP (PDB: 3lz0) showing the locations of the tested 8-oxodGuo. The 8-oxodGuo modifications are represented by means of the red sphere model. b 10% native PAGE depicting the bound of hOGG1K249Q with NCP-8-oxodGuo-137. The red arrow highlights a band corresponding to a 1:1 complex of hOGG1K249Q/NCP-8-oxodGuo-137. c Quantification with fits for the binding of hOGG1K249Q to dsDNA. d Quantification with fits for the binding of hOGG1K249Q to NCPs. The presented data display the individual data points of three independent experiments. NCP nucleosome core particle, SHL superhelical location. Source data are provided as a Source Data file.
