Fig. 3: Distortion of the DNA structure upon hOGG1K249Q binding to the NCP-8-oxodGuo-137.
From: Structural basis for human OGG1 processing 8-oxodGuo within nucleosome core particles
a Alignment of the hOGG1K249Q/NCP-8-oxodGuo-137 complex structure with that of a canonical NCP (PDB: 3lz0). The close-up view highlights the pronounced widening of the minor groove upon hOGG1 binding and the resulting ejection of 8-oxodGuo-137 from the DNA helix. b DNA registry shifts at SHL 5.5 to SHL 7.0 due to local distortions of nucleosomal DNA upon hOGG1 binding. In comparison to a canonical NCP model (PDB: 3lz0), the DNA is pushed inwards from the nearby DNA entry/exit site toward the dyad axis and partially lifted off the NCP core. c Distance plot depicting the minor groove width of the nucleosomal DNA segment spanning from SHL 5.5 to SHL 7.0. The minor groove width was measured between DNA backbone phosphates. Light gray: histone octamer of hOGG1K249Q/NCP-8-oxodGuo-137 complex, dark gray: DNA of hOGG1K249Q/NCP-8-oxodGuo-137 complex, light blue: histone octamer of canonical NCP, dark blue: DNA of canonical NCP, orange: hOGG1. NCP nucleosome core particle. Source data are provided as a Source Data file.
