Fig. 3: Phages belonging to the same CG utilize the same bacterial receptors.
From: A blueprint for broadly effective bacteriophage-antibiotic cocktails against bacterial infections
a Schematic model description of three major surface receptors involved in phage uptake by P. aeruginosa, including Type IV pili (pink), oligosaccharides antigens (OSA) and lipopolysaccharide (LPS, blue), and flagella (green). This schematic model was created in BioRender. Kim, K. (2023) BioRender.com/q97t970. b Representative images of twitching motility assay by PA14, PA14ΔpilA, and PA14 strains exposed to DMS3vir, TIVP-H6, Luz24, and OMKO1 phages. Scale bar = 10 mm. c Suppression Index measured for PA14 (black) and the PA14ΔpilA (pink) by eight different types of phages, each at an MOI of 100. d Colony biofilm phenotypes of PA14, PA14ΔwapR, and the designated phage-exposed strains on Congo red agar medium after 100 hours of growth. Scale bar = 2 mm. e Suppression Index measured for the wild-type PA14 (black) and PA14ΔwapR (blue) by eight different types of phages at an MOI of 100. f Representative images of swimming motility assay by PA14, PA14ΔfliC, and PA14 strains exposed to DMS3vir, TIVP-H6, Luz24, and OMKO1 phages. Scale bars = 10 mm. g Suppression Index measured for PA14 (black) and PA14ΔfliC (green) by eight different types of phages, each at an MOI of 100. Images shown in b, d, and f are based on a minimum of triplicate independent replicates. All reported values for c, e, and g are depicted from three independent experiments with n = 3 and associated error bars represent standard deviation from the mean. Statistical significance in c, e, and g is derived from Two-way analysis of variance (ANOVA) and significance levels include *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Source data are provided as a Source Data file.
