Fig. 4: Ancestral holozoan and animal Sox factors can induce pluripotency in mice.
From: The emergence of Sox and POU transcription factors predates the origins of animal stem cells
a Section of the maximum likelihood phylogeny of holozoan Sox HMG domains used for ancestral sequence reconstruction. Colored spheres mark reconstructed nodes. The full phylogeny is shown in Supplementary Fig. 1. b Sequence alignment showing selected key residues of the ancestral Sox HMG domains reconstructed from the phylogeny shown in (a). The arrow marks residue 57, which is critical for the selective pairing with Oct433. c Representative microscope images of iPSC colonies on day 14. Scale bar, 80 μm. d Quantification of iPSC reprogramming efficiency normalized to the colony numbers of mSox2 on day 14 (n = 5 technical replicates in total, including 2 biological replicates each with 2 technical replicates and 1 biological replicates). The box displays the interquartile range, with the left edge representing the lower quartile (25th percentile) and the right edge indicating the upper quartile (75th percentile). The median value is shown as a line splitting the box. Source data and statistics are provided as a Source Data file.
