Fig. 2: Reduced diversity in BCR repertoire of PAPS patients. | Nature Communications

Fig. 2: Reduced diversity in BCR repertoire of PAPS patients.

From: Defective germinal center selection results in persistence of self-reactive B cells from the primary to the secondary repertoire in Primary Antiphospholipid Syndrome

Fig. 2: Reduced diversity in BCR repertoire of PAPS patients.

A Schematic of peripheral B cell isolation from PAPS (n = 10) and HD (n = 7) blood followed by CITE seq including single-cell transcriptomic, antibody repertoire sequencing and ADT. VDJ sequencing was used to obtain paired heavy- and light-chain V(D)J with an average of 5,231 cells recovered per library. B–D IGHV (B), IGKV (C), and IGLV gene (D) usage frequencies in peripheral B cells from PAPS patients (red) and HD (blue). E, F IGHD families (E) and IGHJ gene (F) usage frequencies in peripheral B cells from PAPS patients (red) and HD (blue). G, H CDR3 length (G) and somatic hypermutations per VH segment (H) (nucleotide exchanges compared with the nearest germline gene segment). I, J Chao1 (I) and D50 diversity index (J) according to isotype in PAPS patients (red) and HD (blue). For IGHA diversity, n = 9 in patient group, as one patient did not have IgA+ B cells. Data are presented as mean values ±SEM. Significance was determined using a Two-tailed Mann–Whitney test. ADT antibody derived tag, HD healthy donor, PAPS primary antiphospholipid syndrome.

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