Fig. 6: Peripheral B cells from PAPS patients exhibit mTORC1 and MYC transcriptomic signature. | Nature Communications

Fig. 6: Peripheral B cells from PAPS patients exhibit mTORC1 and MYC transcriptomic signature.

From: Defective germinal center selection results in persistence of self-reactive B cells from the primary to the secondary repertoire in Primary Antiphospholipid Syndrome

Fig. 6: Peripheral B cells from PAPS patients exhibit mTORC1 and MYC transcriptomic signature.

A UMAP projection and clustering of 78,355 B cells analyzed by scRNA-seq. B Bar plot showing cell frequencies from PAPS patients (n = 10) and HD (n = 7) that contributed to cluster c1 to c14. C UMAP projection showing six annotated B cell subtypes. D Plot showing the top 10 GSEA signaling pathway significantly enriched in total B cells from PAPS patients as opposed to HD. E Plots depicting the enrichment of MYC and mTORC1 GSEA signaling pathways in naive and memory B cells from PAPS patients as opposed to HD. F Heatmap listing top genes of mTORC1 signaling pathway enriched in PAPS patients. G Projected enrichment score of indicated pathway for each cell onto UMAP from APS patients. The darker shading represents higher enrichment. H Dot plot for expression of B cell cytokine receptors among naive clusters in all samples. Colors represent minimum-maximum normalized mean expression, and sizes indicate the proportion of cells expressing respective genes. I TACI MFI on naive B cells from two representative APS and two HD (left); TACI and BAFF MFI ratio on naive B cells from APS patients as opposed to HD (right) using FCM. Data are presented as mean values ±SEM. Significance was determined using a Two-tailed Mann–Whitney test. FCM flow cytometry, FDR False Discovery Rate, GSEA Gene Set Enrichment Analysis, HD healthy donor, PAPS primary antiphospholipid syndrome.

Back to article page