Fig. 3: CARS-ISM images of HEK cells.
From: Super-resolved coherent anti-Stokes Raman scattering microscopy by coherent image scanning
CARS intensity images of HEK cells. a and g basic CARS image of a large field of view (15 × 15 μm) of several cells with squares marking the region imaged in b–f and h–l, respectively. The intensity ∣ECARS∣2 is plotted in b and h by considering straightforward integration of the signal from all detector pixels (basic CARS); c and i the confocal image retrieved from the central pixel of the detector; d and j the ISM image obtained from performing pixel reassignment on the field amplitude captured by each detector pixel; e and k the ISM image following Fourier reweighting analysis. All intensity figures are normalized to the same total intensity and plotted on the same color scale. Additionally, the phase images corresponding to the ISM images are shown in (f) and (l). m and n show line cuts from the cells in a–f and g–l, respectively, comparing open-aperture, confocal, ISM, and FR-ISM. Excitation power before the objective is Ppump = 4.8 mW and PStokes = 2.8 mW. Pixel dwell time is ~40 ms per modulation phase. The intensity image insets in m and n are scaled differently to show detail. Scale bar = 1 μm.
