Fig. 2: Severe (vs moderate) hypoxic episodes evoke greater spinal adenosine accumulation.
From: Microglia regulate motor neuron plasticity via reciprocal fractalkine and adenosine signaling
A Adenosine/inosine probes were placed between ventral C3/C4 to measure changes in adenosine accumulation during hypoxia. B Average traces of extracellular adenosine concentration (µM) during 5 min of moderate (PaO2 = 42.7 ± 2.0 mmHg) or severe (PaO2 = 27.2 ± 0.8 mmHg) hypoxia (n = 5 independent recordings per group, collected in 3 rats). Measurements of technical replicates allowed appropriate ‘washout’ time between subsequent hypoxic exposures for extracellular adenosine to return to baseline levels, consistent with published data from our laboratory38. Measurements between biological and within technical replicates did not differentiate more than one standard deviation from each other (see Source Data). Greater adenosine accumulation was observed in severe hypoxic episodes when expressed as C peak adenosine level ([ADO]peak; t(8) = −5.299, p < 0.001; unpaired t-test, two-sided) or D total area under the curve ([ADO]AUC; t(8) = −4.218, p = 0.003; unpaired t-test, two-sided). E PaO2 strongly correlates with measured extracellular adenosine levels (F(1,14) = 206.099, p < 0.0001; nonlinear regression ANOVA; r = 0.9677, r2 = 0.9364, adjusted r2 = 0.9318; nonlinear regression). Adjustments were made for multiple comparisons. Bars are means ± SEM. Source data are provided as a Source Data file. Figures created in BioRender. Marciante, A. (2024) https://BioRender.com/e55f542.
