Fig. 8: Stop-flow enhances AAV-sized gold nanoparticle uptake. | Nature Communications

Fig. 8: Stop-flow enhances AAV-sized gold nanoparticle uptake.

From: AAV delivery strategy with mechanical support for safe and efficacious cardiac gene transfer in swine

Fig. 8: Stop-flow enhances AAV-sized gold nanoparticle uptake.The alternative text for this image may have been generated using AI.

a, b Images are representative of gold nanoparticle detection (a) 30 min after Continuous vs Stop-flow delivery and (b) 4 h after Stop-flow delivery in remote (mid-posterior) and infarct (mid-anterior) tissues. Gold nanoparticles were not detected after Continuous delivery but were found with Stop-flow delivery (gold arrows), either attached at the endothelium-lumen interface or in vesicular structures. Images acquired at 4000x-6000x direct magnification were fitted to the same scale. Scale bar in (a), larger images = 600 nm; scale bar in (a) projections, (b) = 200 nm. c Observed instances of gold nanoparticle detection over equivalent grid areas and imaging time were recorded in each condition (n = 1 animal per condition, with duplicate tissues from each tissue region) representing gold nanoparticle distribution.

Back to article page