Fig. 2: ENT1 and A1R expression in DRG and spinal cord neurons.
From: Design of an equilibrative nucleoside transporter subtype 1 inhibitor for pain relief
a, b RNA scope in situ hybridization showing ENT1 transcript (SLC29A1) expression in the DRG (a) and spinal cord dorsal horn (b) of mice. The dorsal horn top edge is indicated by the dotted line. Scales, 50 μm in a and 100 μm in b. c Double immunostaining showing co-localization of ENT1 and A1R in the dorsal horn and ventral horn (box size, 250 × 250 μm). Right panels, enlarged images from the boxes. Scale, 25 μm. Experiments in a–c were repeated 2–3 times. d Left, RNAscope in situ hybridization images showing co-localization of SLC29A1 and ADOR1 (A1R) in human DRG (hDRG) neurons. The same DRG sections were co-stained with NeuN antibody (neuronal marker) and DAPI (nuclei marker). Arrows indicate neurons that are positive for both ENT1 and A1R. Scale, 50 μm. Right, quantification showing the ratio and percentage of SLC29A1 or/and ADOR1 positive and negative DRG neurons from four human donors.
