Fig. 4: JH-ENT-01 reduces formalin-induced acute inflammatory pain.
From: Design of an equilibrative nucleoside transporter subtype 1 inhibitor for pain relief
a–c Effects of intrathecal (i.t.) injection of the ENT1 inhibitors JH-ENT-01 (1, 10, and 100 nmols) and dilazep (100 nmol) on formalin-induced inflammatory pain in phase 1 (0–10 min) and phase 2 (10–45 min) in male mice (a), female mice (b), and males and females combined (c). Top panels show the time course of formalin-induced pain. n = 4 per sex; n = 8 for both sexes. The inhibitors were given 30 min prior to the intraplantar injection of formalin (5%, 20 μl). d Rotarod tests before and 60 min after i.t. delivery of 100 nmol of JH-ENT-01 and dilazep. n = 6 animals per group with mixed sexes. Two-way ANOVA followed by Bonferroni posthoc comparison. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ns, not significant. All data were expressed as mean ± s.e.m.
