Fig. 1: Mcm10 protects against (GAA)n repeat instability.
From: Stabilization of expandable DNA repeats by the replication factor Mcm10 promotes cell viability
a Overview of Mcm10 functions during DNA replication. Created in BioRender. Masnovo, C. (2024) https://BioRender.com/g31r060. b Genetic assay system to measure repeat expansion rates. Created in BioRender. Masnovo, C. (2024) https://BioRender.com/q07v344. c Genetic assay system to measure repeat contraction rates. Created in BioRender. Masnovo, C. (2024) https://BioRender.com/q07v344. d Expansion rates in the mcm10-1 mutant at the permissive (23 °C) and semi-permissive (27 °C) temperatures. Plotted values indicate the corrected rate calculated with FluCalc (https://flucalc.ase.tufts.edu/)66 and the error bars represent 95% confidence intervals (see Source Data File). Numbers within bars indicate fold increase over the respective wild-type value. Expansion rates were determined by PCR of at least 96 FOAR colonies derived from two biological replicates. An event was considered an expansion when at least 10 repeats were added. e Contraction rates for the mcm10-1 mutant at the permissive (23 °C) and semi-permissive (27 °C) temperatures. Plotted values indicate the corrected rate calculated with FluCalc (https://flucalc.ase.tufts.edu/)66 and the error bars represent 95% confidence intervals (see Source Data File). Each experiment was conducted using two biological replicates.
