Fig. 2: A suppressor mutation in the Mcm2 subunit of the CMG helicase rescues instability of mcm10-1.
From: Stabilization of expandable DNA repeats by the replication factor Mcm10 promotes cell viability
a Expansion rates in the mcm10-1 and mcm2G400D mutants at the semi-permissive temperature (27 °C). RPA OE indicates RPA overexpression via 2μ multicopy plasmid containing all three RPA genes: RFA1, RFA2 and RFA3. Plotted values indicate the corrected rate calculated with FluCalc (https://flucalc.ase.tufts.edu/)66 and the error bars represent 95% confidence intervals (see Source Data File). Each experiment was conducted with two biological replicates. Numbers within bars indicate fold increase over the respective wild-type value. b Contraction rates in the mcm10-1 and mcm2G400D mutants at the semi-permissive temperature (27 °C). Plotted values indicate the corrected rate calculated with FluCalc (https://flucalc.ase.tufts.edu/)66 and the error bars represent 95% confidence intervals (see Source Data File). Each experiment was conducted with two biological replicates. Numbers within bars indicate fold increase over the respective wild-type value. c Western blot of Pol1 (Cdc17) tagged with a 3xFLAG tag in WT and mcm10-1 strains at the semi-permissive (30 °C) and at the restrictive (37 °C) temperatures. The experiment conducted twice using independent biological replicates (see Source Data file). Ponceau S staining was used as a control for total protein loading.
