Fig. 8: Type IV Filament Systems and Field-Emission Scanning Electron Microscopy. | Nature Communications

Fig. 8: Type IV Filament Systems and Field-Emission Scanning Electron Microscopy.

From: Respiratory processes of early-evolved hyperthermophiles in sulfidic and low-oxygen geothermal microbial communities

Fig. 8

Highly expressed type four filament (Tff) systems in Thermocrinis MAGs (A) likely explain the extensive network of pili-like structures (~20–25 nm diameter) commonly observed in filamentous streamer communities from Conch Spring (B) and Octopus Spring (C). [Abbreviations and Definitions: Bechler 2008 = Thermocrinis entry from Bechler Spring, YNP24. Arrows in C indicate Tff structures versus cells. [pilA = Type IV pilus assembly, pilW = Type IV pilus assembly, pulG = Type II secretory pathway, fimT = Type IV fimbrial biogenesis, pilY = adhesin; nfuA = Fe-S biogenesis, pilQ = pilus secretin, recJ = ssDNA exonuclease, HCO = heme copper oxidase complex subunits I, II, and III. Source data are provided as a Source Data file. Micrographs shown in B and C were chosen from a large collection of over 30 replicates from 3 different sample years and a minimum of 10 replicate images per year. Additional FE-SEM micrographs are provided in Supplementary Fig. 14. Complete tables of observed transcripts and expression levels are provided in Supplementary Data 1–3].

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