Fig. 2: Ribosomes are associated with the mother cell and forespore peptidoglycan and membrane proteins. | Nature Communications

Fig. 2: Ribosomes are associated with the mother cell and forespore peptidoglycan and membrane proteins.

From: Ribosomes translocation into the spore of Bacillus subtilis is highly organised and requires peptidoglycan rearrangements

Fig. 2

a Polysome profiles of the DSP cross-linked (DSP) and non-cross-linked (D) samples of sporulating B. subtilis cells lysed with digitonin. Fractions analysed by MS, in both DSP and D conditions, are highlighted in grey: monosome (fr1), monosomal shoulder (fr2) and disome (fr 3 and 4). Positions of the ribosomal subunits (30S, 50S), monosome (70S) and polysomes are indicated. b Volcano plots illustrating the mass spectrometry proteomics data for cross-linked vs non-cross-linked samples (DSP vs D), for the consecutive fractions 1–4. The x-axis shows the calculated Log2 fold change and p values are represented on the y-axis as −Log10(p value) (Student’s T-test, two-sided; no adjustments for multiple comparisons). The vertical and horizontal dotted lines show the cut-off of Log2(Fold Change) = ± 1 (Fold Change = ± 2), and for p value = 0.05, respectively. The enriched proteins are categorised into 11 functional groups, colour-coded accordingly to the legend shown in bottom left. Protein components of the feeding tube channel (SpoIIIAH-AG, SpoIIQ and GerM) and peptidoglycan degradation module (SpoIIP and SpoIIB) are labelled. Source data are provided as a Source Data file.

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