Fig. 6: FvSEP3 regulates fruit ripening downstream of FvALKBH10B.

a Gene expression of FvSEP3 as determined by RT-qPCR. RT root, ST stem, LE leaf, FL flowers, SG small green, LG large green, WF white fruit, TF turning fruit, RF red fruit. ACTIN was used as an internal control. b Representative images of fruits silencing FvSEP3 in the Fvalkbh10b mutant background. Fruits at large green stage were used for agroinfiltration and considered as days 0. EV empty vector, WT wild type. Scale bar, 0.5 cm. c The mRNA levels of FvSEP3 in fruits shown in (b) as determined by RT-qPCR. d Anthocyanin contents in the fruits shown in b. e, Representative images of fruits overexpressing FvSEP3 in the Fvalkbh10b mutant background. Fruits at large green stage were used for agroinfiltration and considered as days 0. Scale bar, 0.5 cm. f The mRNA levels of FvSEP3 in fruits shown in (e), as determined by RT-qPCR. g Anthocyanin contents in the fruits shown in (e). h RNA immunoprecipitation (RIP) assay showing that FvALKBH10B directly binds FvSEP3 transcript. The FvALKBH10B-HA fusion protein was transiently expressed in fruits of Fvalkbh10b mutant, and then the FvALKBH10B-bound mRNAs were immunoprecipitated and submitted to RT-qPCR assay. For a, c, d, and f–h, values are means ± SEM (n = 3 biological replicates). For c, d, f, and g, data are analyzed using one-way ANOVA with Tukey’s HSD test (P < 0.05). Different lowercase letters indicate significant differences. For h, asterisks indicate significant differences (unpaired two-sided t-test; ***P < 0.001). Source data are provided as a Source Data file.