Fig. 2: Genomic methylation of F1 oocytes.
From: Gestational diabetes mellitus causes genome hyper-methylation of oocyte via increased EZH2
a Genomic methylation of GV oocytes is examined using immunofluorescence, and b the relative fluorescence intensity of 5mC is examined using Image J, NGDF1, n = 33; GDF1, n = 24, p = 0.000322; c the methylation level of 5mC and 5hmC in MII oocytes is also examined using immunofluorescence, and d, e the relative fluorescence intensity is calculated using Image J;; n: NGDF1 = 47 and GDF1 = 41 for 5mC (p = 0.027547), and NGDF1 = 44 and GDF1 = 39 for 5hmC (p = 0.022106); f 5mC/5hmC ratio is calculated; n: NGDF1 = 37 and GDF1 = 35, p = 0.001764; g proportion of different mC types including CG (p = 0.03063), CHG (p = 0.34116), and CHH (p = 0.13043), H = A, T, or C. h genomic methylation of F1 MII oocytes is further examined using whole genome bisulfite sequencing, genomic methylation level of GC for each sample is presented (p = 0.013026). data presented as mean ± SEM. *p < 0.05, ***p < 0.001. Bar, 20 μm. 5mC, methylated cytosine; 5hmC, hydroxymethylcytosine; PI, prodium lodide; DAPI, 4’,6-diamidino-2-phenylindole; Source data are provided as a Source Data file.
