Fig. 1: S100P is positively correlated with ferroptosis resistance in liver cancers.

a The ferroptotic sensitivity assessment of a panel of HCC cell lines (HuH7, HepG2, Hep3B, Li7, SNU387) using CCK-8 after the treatment of indicated concentrations of RSL3 for 12 h. b Cell death of HuH7 and SNU387 cells treated with RSL3 (200 nM) and fer-1 (2 µM) for 6 h. Dead cells were stained by Sytox Green. c Lipid peroxidation measurements of HuH7 and SNU387 cells treated with RSL3 (200 nM) and lipro-1 (2 µM) for 3 h. d Heat maps of differently expressed genes from CCLE database in HuH7 and SNU387 cells. e The relative mRNA level of PTGS2 and S100P in HuH7 cells treated with RSL3 (2 µM) and SNU387 cells treated with RSL3 (200 nM) for indicated times. f The expression of S100P of HuH7 cells treated with RSL3 (1 μM) for indicated times. g The expression of S100P of HuH7 cells treated with erastin (5 μM) for indicated times. The Western blot experiments were repeated three times independently with similar results in f and g. Data and error bars are mean ± s.d., n = 3 biological independent experiments in a–c and e. P values were calculated using two-tailed Student’s t test in b, c and e. Source data are provided as a Source Data file.