Fig. 2: S100P is a ferroptosis suppressor.

a, c Western blot analysis of HuH7 or HepG2 cells expressing sg-ctrl and sg-S100P. b, d Cell death of HuH7 or HepG2 cells derived from a or c treated with RSL3 (2 µM) and fer-1 (2 µM) for 6 h. e Lipid peroxidation of HuH7 and S100P KO cells treated with RSL3 (2 µM) and lipro-1 (2 µM) for 3 h. f Western blot analysis of S100P expression in Huh7 S100P KO cells with ectopic expression of S100P. g Cell death of Huh7 cells derived from f treated with RSL3 (2 µM) and fer-1 (2 µM) for 6 h. h Western blot analysis of SNU387 cells transfected with S100P. i Cell death of SNU387 cells derived from h treated with RSL3 (200 nM) and fer-1 (2 µM) for 6 h. j Cell death assay of SNU387 cells over-expressing S100P treated with erastin (2 µM) and fer-1 (2 µM) for 24 h. k Representative images of 3D Spheroids derived from HuH7 and S100P KO cells with the treatment of IKE (5 µM) and lipro-1 (2 µM) for 48 h. Scale bars, 10 µm. l Cell death of 3D Spheroids visualized by Sytox green positive area. m Cell death of attached and detached cells (anoikis model constructed in HuH7 cells) treated with RSL3 (2 µM) for 6 h. n Western blot analysis of S100P expression in HuH7 attached and detached cells treated with RSL3 (1 µM) for indicated times. o Anoikis model construction of HuH7 and S100P KO cells treated with RSL3 (2 µM) for 6 h. Scale bars, 10 µm. p Cell death of anoikis model constructed in HuH7 and S100P KO cells treated with RSL3 (2 µM) for 6 h. The Western blot experiments were repeated three times independently with similar results in a, c, f, h and n. Data and error bars are mean ± s.d., n =  3 biological independent experiments in d, e, g, i, j, l, m and p. n = 4 biological independent experiments in b. Statistical analysis was done with two-tailed Student’s t test. Source data are provided as a Source Data file.