Fig. 3: S100P resists ferroptosis independent of RAGE and classical ferroptosis genes.

a Cell viability of SNU387 S100P OE cells pretreated with indicated concentrations of Cromolyn for 12 h and then treated with RSL3 (200 nM) for 6 h. b Cell viability of HT1080 S100P OE cells pretreated with indicated concentrations of Cromolyn for 12 h and then treated with RSL3 (200 nM) for 6 h. c Western blot analysis of S100P OE cells of SNU387 expressing sg-ctrl and sg-RAGE. d Cell death measurements of SNU387 S100P OE cells expressing sg-ctrl or sg-RAGE with the treatment of RSL3 (100 nM) for 6 h. e Western blot analysis of HT1080 S100P OE cells expressing sg-ctrl and sg-RAGE. f Cell death measurements of HT1080 S100P OE cells expressing sg-ctrl or sg-RAGE with the treatment of RSL3 (100 nM) for 6 h. g Western blot analysis of classical ferroptosis-related genes in HuH7 and S100P KO cells. h Western blot analysis of classical ferroptosis-related genes in HepG2 and sg-S100P cells. i Western blot analysis of classical ferroptosis-related genes in SNU387 and S100P OE cells. j Western blot analysis of classical ferroptosis-related genes in HT1080 and S100P OE cells. The Western blot experiments were repeated three times independently with similar results in c, e and g–j. Data and error bars are mean ± s.d., n =  3 biological independent experiments in a, b, d and f. Statistical analysis was done with two-tailed Student’s t test in d and f. Source data are provided as a Source Data file.