Fig. 5: NORAD downregulation does not induce ISG expression in mouse cells. | Nature Communications

Fig. 5: NORAD downregulation does not induce ISG expression in mouse cells.

From: lncRNA NORAD modulates STAT3/STAT1 balance and innate immune responses in human cells via interaction with STAT3

Fig. 5: NORAD downregulation does not induce ISG expression in mouse cells.

a qPCR analysis of Norad KD in MEF cells demonstrating that KD of Norad does not change the RNA levels of Stat3, Stat1, and Isg15 (n = 2). b A volcano plot of RNA-seq data from Norad KD versus control siRNA in MEF cells at 24 h post-transfection. Differentially expressed genes (Padj < 0.05, -2 > FC > 2) are in bold, ISGs are marked in red (n = 3). c The FCs of differentially expressed genes upon NORAD KD in HFFs compared to hESCs (left) and HFFs compared to MEFs (right) showing a high correlation between the undifferentiated and the fully differentiated human cells while no significant changes are detected in gene expression in MEFs. ISGs are marked in red. d CLIP-qPCR validation of STAT3 binding to NORAD in hESCs, HFFs and MEFs (n = 2). As demonstrated, there is no interaction between STAT3 and Norad in the mouse cells. Source data are provided as a Source Data file.

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