Fig. 6: The presence of an Alu element in the NORAD transcript in primates is associated with NORAD-STAT3 binding. | Nature Communications

Fig. 6: The presence of an Alu element in the NORAD transcript in primates is associated with NORAD-STAT3 binding.

From: lncRNA NORAD modulates STAT3/STAT1 balance and innate immune responses in human cells via interaction with STAT3

Fig. 6: The presence of an Alu element in the NORAD transcript in primates is associated with NORAD-STAT3 binding.

a Evolutionary conservation across the NORAD transcript, depicting weak sequence conservation at the STAT3 binding site. STAT3 binding site, Alu and NORAD repeats are illustrated in gray rectangles. b Phylogenetic tree based on the sequence of the STAT3 binding site (135 nts). RNA predicted structures (predicted by RNAfold) are presented. Bootstrap values above 0.7 (calculated based on 1000 trees) are represented in circles. Red, Blue and green colored branches depict sequences derived from primates containing Alu in NORAD, primates not encoding Alu and other mammals, respectively. c Heatmap of RMSD values representing pairwise structural comparisons of the predicted 3D structures of the STAT3 binding region on NORAD from the 30 organisms shown in supplementary Fig. 5b. The structural models were built based on the sequences around the core region, centered around the loop. d EMSA results for STAT3 binding to predicted binding site in Human (red), Rhesus (pink), Ma’s Night Monkey (blue) and Mouse (green) (n = 2). Source data are provided as a Source Data file.

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