Fig. 6: WRN-WT and WRN-6A unwinding of TP-G4 DNA substrate. | Nature Communications

Fig. 6: WRN-WT and WRN-6A unwinding of TP-G4 DNA substrate.

From: Phosphorylation-dependent WRN-RPA interaction promotes recovery of stalled forks at secondary DNA structure

Fig. 6

a Helicase reactions containing radiolabeled tetramolecular parallel TP-G4 substrate (0.25 nM) and increasing concentration of WRN-WT and WRN-6A proteins (0.5, 1, 2, 4, 8 and 16 nM). b Evaluation of helicase activity of WRN-WT (2 nM) and WRN-6A (2 nM) with increasing amount of RPA (1, 2, 4, 8, 16 nM). NE indicates no enzyme control. M: marker of single-stranded TP-G4 oligonucleotide. Autoradiographs are representative of two independent replicates. Source data are provided as a Source Data File.

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