Fig. 4: Effect of optical aberrations and noise on deblurring performance using simulations.
a Example of optical aberrations in real microscopy system (spatially-varying PSF in mesoSPIM23). b Example of noise in real microscopy system (low-light condition in dSLAM53). c Ground truth image for simulation on effect of optical aberrations and noise. Four different levels of optical aberrations and noise with the corresponding raw images (d Shift-invariant PSF (ideal case); e Shift-variant PSF; f Shift-variant PSF with rotations along z-direction; g Shift-variant PSF with rotations along z-direction and noise across the entire imaging volume). h–k Comparison of Self-Net, CARE, and SSAI-3D on deblurring performance using SSIM in scenarios d–g. Bottom: Blow-ups of the co-registered images within the white box. Scale bars: 2 mm (a); 25 µm (b–k).
