Fig. 5: Pseudobulk differentially expressed genes (DEGs).

a Barplot representation of the number of pseudobulk DEGs calculated for each pathology in comparison to the control population. Upregulated DEGs were calculated using the complete HuMicA object and subsets consisting of Homeos subpopulations (clusters 0, 1, 4), DAMs (clusters 1, 3, 5, 6) and DIMs (cluster 2). Significant DEGs are considered for an adjusted p value (FDR) inferior to 0.05 and log2FoldChange superior to 1. b Heatmap representation of the significance of the overlap between the lists of pseudobulk DEGS, calculated with the GeneOverlap function. Overlaps were calculated between each pathology within each subset (HuMicA, Homeos, DAMs, DIMS). The degree of significance is depicted by the Jaccard index and a p-value inferior to 0.05 is highlighted by a white asterisk. c Dot plot representation of the gene ontology (GO) enrichment of all lists of pseudobulk DEGs between each pathology and controls accounting for the complete HuMicA object and subsets consisting of Homeos subpopulations (clusters 0, 1, 4), DAMs (clusters 1, 3, 5, 6), DIMs (cluster 2) and MAC (cluster 7). Selected statistically significant GO terms are represented (adjusted p-value (Benjamini-Horchberg) < 0.05). Significance is represented by the log of the fold change and the negative of the log of the adjusted p-value (FDR). Source data are provided as a Source Data file.