Fig. 2: MHV68 latency establishment is enhanced in p53-deficient mice. | Nature Communications

Fig. 2: MHV68 latency establishment is enhanced in p53-deficient mice.

From: Intrinsic p53 activation restricts gammaherpesvirus driven germinal center B cell expansion during latency establishment

Fig. 2

a Flow cytometry analyses to determine the percentage of infected splenocytes (YFP +) for each mouse genotype at 16 days after mock infection or infection with 104 PFU of H2B-YFP MHV68. Data represent means +/− SEM for 2-3 independent infections with a minimum of 3 mice per group. Each dot represents an individual mouse, n = 4-5. Welch’s two-sided t test, **p < 0.005. b Quantification of latently infected splenocyte frequencies by limiting-dilution PCR on day 16 post-infection. p53-/- or p53+/+ mice were infected intranasally with 104 PFU of H2B-YFP MHV68. Data represent means +/− SEM for 2 independent infections with a minimum of 3 mice per group. Extra sum-of-squares F test p < 0.0001, F = 40.36 (1, 22). c Quantification of latent MHV68 reactivation efficiency from infected p53-/- or p53+/+ splenocytes on day 16 post-infection. Reactivation was detected by scoring cytopathic effect in a limiting-dilution ex vivo culture. Data represent means +/− SEM for 2-3 independent infections with a minimum of 3 mice per group. Extra sum-of-squares F test p = 0.0024, F = 10.36 (1, 46). Replicate graphs in Supplementary Fig. 3. d table of values from (a, and c). e, Immunohistochemical visualization of B cells (B220+) and MHV68 infected cells (YFP+) in spleen sections from p53-/- or p53+/+ mice on day 16 after infection. Representative images of splenic follicles are shown. Scale bars indicate 100 μm. Additional representative images in Supplementary Fig. 4.

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