Fig. 6: Functional testing of medulloblastoma-associated candidate peptides revealed immune recognition by non-autologous CD8 + T cells.
From: Mapping naturally presented T cell antigens in medulloblastoma based on integrative multi-omics
a Workflow of non-autologous CD8 + T cell priming with artificial antigen-presenting cells. T cell responses were defined via tetramer staining and were considered positive when a 3-fold increase compared to the negative control and >1% of tetramer-positive cells among CD8 + T cells was observed. Created in BioRender. Neidert, M. (2021) BioRender.com/w57d406. b Left column (negative control): medulloblastoma-specific tetramer staining of CD8 + T cells primed with an unrelated, HLA-matched peptide. Right column: Positive tetramer staining of CD8 + T cells primed with DCLK1/287-96, WNT5A20-28, and CMKKLR181-90 demonstrating a significant antigen-specific response. Source data are provided as a Source Data file.
