Fig. 4: Stroma drives tumour immune exclusion in MIRO.
From: Micro Immune Response On-chip (MIRO) models the tumour-stroma interface for immunotherapy testing
a Co-culture timeline in MIRO in the absence or presence of stroma. b Time projection of time-lapse imaging of immune cells reaching MIRO inlet and interacting with the tumour spheroid. For each time point, the fluorescence image of the immune cells was binarized and overlaid with the tumour cell fluorescent signal. Immune cells overlapping with tumour cells over time are displayed in yellow, the others are shown in cyan, and BCC are in red. Scale bar: 100 μm. c Quantification of immune cells infiltrated in the tumour compartments in the absence (without CAF#1 black dots) and presence (with CAF#1, green dots) of CAF#1. The dotted line represents the mean value of the immune cell counted over time; SD is shown as a filled coloured area (n = measures, with stroma n = 8; without stroma n = 6, from at least 3 independent experiments;), unpaired t-test over the last 10 time points (two-tailed) was performed, ****p < 0.0001. Source data are provided as a Source Data file.
