Fig. 2: Effects of Neo-EVs, AR-Neo-EVs, and Adu-EVs on angiogenesis of Human umbilical vein endothelial cells (HUVECs) in vitro.
From: Cardiac repair using regenerating neonatal heart tissue-derived extracellular vesicles
a PKH26-labeled EVs were co-cultured with HUVECs for 6 h. Endocytosed EVs (PKH26, red) can be seen within the cytoplasm of HUVECs (CD31, green). Scale bar = 5 μm. b Tube formation experiments of HUVECs treated with different EVs. Scale bar = 150 μm. c–e Quantitative analysis of tube formation experiments (n = 5 independent experiments). ***p < 0.001 vs. Ctl, ***p < 0.001 vs. Neo-EVs. f Scratch migration experiments of HUVECs treated with different EVs. Scale bar = 150 μm. g Quantitative analysis of scratch migration experiments (n = 5 independent experiments). ***p < 0.001 vs. Ctl, ***p < 0.001 vs. Neo-EVs. One-way ANOVA followed by Tukey’s Multiple Comparisons test (c–e). Two-way ANOVA followed by Tukey’s Multiple Comparisons test (g). Error bars represent the mean ± SEM of each group. Source data are provided as a Source Data file. Exact p values are provided in the Source Data file.
