Fig. 6: Viability and ATP content of Hydrogenobacter RSW1 cells when incubated in the presence and absence of an electron donor (H2) or an electron acceptor (S0).
From: Simultaneous aerobic and anaerobic respiration in hot spring chemolithotrophic bacteria
Cell concentration and cell viability (panel A) were quantified in cultures grown with hydrogen (H2) and elemental sulfur (S0), with S0 only, or with H2 only over 4 days of incubation. The production of cells and their viability was determined using live/dead staining and fluorescent microscopy (panel A). ATP content of cells following 4 days of incubation as determined using a firefly luciferase assay (panel B). Individual replicates are shown as black circles. All cultures were provided with 150 μM SO42- as a sulfur source and were incubated on their sides at 70 °C on a shaking incubator (50 revolutions per minute). The average and standard deviation of measurements of triplicate cultures is indicated. Abbreviations: BD, below detection. Source data are provided as a Source Data file.
