Fig. 2: Manufacturing Data. | Nature Communications

Fig. 2: Manufacturing Data.

From: Cultivated autologous limbal epithelial cell (CALEC) transplantation for limbal stem cell deficiency: a phase I/II clinical trial of the first xenobiotic-free, serum-free, antibiotic-free manufacturing protocol developed in the US

Fig. 2: Manufacturing Data.

A Schematic representation of CALEC preparation from the limbal biopsy of the donor eye, harvesting, and isolation of limbal epithelial cells into single cell suspension at P0; followed by cell seeding onto the denuded human amniotic membrane, AmnioGraft, draped over a transwell into the secondary (P1) culture. The resulting CALEC construct is then transplanted on the diseased eye (1). Phase contrast images cultivated autologous epithelial cells at P0 (2) and P1(3), Scale – 100 µm. B Spearman Correlation of Colony Forming Efficiency (CFE)% at P0 with Intracellular Adenosine Triphosphate (iATP) ratio (N = 11). C Spearman Correlation of Colony Forming Efficiency (CFE) % with Days from Biopsy to P0 Harvest (N = 13).Includes data for all conforming constructs (N = 14 biopsies). Missing values are detailed in Supplementary Table 3.

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