Fig. 3: BCLAF1 is an oncogene that contributes to p85β oncogenicity.
From: p85β acts as a transcription cofactor and cooperates with BCLAF1 in the nucleus
A–C DOV13 stable cells expressing vector or PIK3R2 (R2-OX) were transfected with BCLAF1 siRNA for 72 h, followed by (A) western blotting for the knockdown efficiency with ERK2 as loading control, or (B) cell migration assay, or (C) cell invasion assay. D–F OVCAR8 cells were transfected with PIK3R2 siRNA or BCLAF1 siRNA alone or in combination for 72 h. Cells were harvested for (D) western blotting, (E) cell migration or (F) invasion assay. G, H DOV13 stable cells expressing vector or PIK3R2 (R2-OX) were transfected with BCLAF1 siRNA. Cells were subjected to (G) cell viability assay or (H) cell cycle analysis. I–L DOV13 and OVCAR8 cells stably expressing BCLAF1 (BCLAF1-OX) or vector control were harvested for (I) western blotting, (J) cell viability assay, (K) cell migration assay, or (L) invasion assay. Assays in (G, J) were done in triplicates and data are shown as mean ± SD. The numbers of cells on the transwell membrane were counted in 5 random fields and data represent mean ± SD (B, C, E, F, K, L). Data and images shown are representative of 3 independent experiments, except data in H which shows the mean of 3 independent experiments ± SD. The numbers below the western blots are densitometry values normalized to the loading control. Scale bar, 200 μm (B, C, K, L); 100 μm (E, F). The P-values shown were calculated using two-way ANOVA with Tukey’s post hoc test (B, C, E–H) or two-tailed t-test (J–L). Source data are provided as a Source Data file.
