Fig. 6: Multi-omics analysis of the FFA pathway in B. minor under different conditions.

a Bar charts showing the total content of C18- and C16- FFA in B. minor under different conditions. b Bar charts showing the content of unsaturated fatty acids and saturated fatty acids in B. minor under different conditions. Pie charts illustrate relative distributions of unsaturated and saturated FAs of B. minor in the respective condition. c Bar charts showing the content of major C16- FFAs. The top right corner bar charts show the proportion of each type of C16- FFA under different conditions. d Bar charts show the contents of major C18- FFAs. The top right corner shows the proportion of each type of C18- FFA in different environments. The plots showed in a-d are using average value of three biological replicates, error bars represent standard error, letters (abc) and asterisks denote significant differences (t-test, P < 0.05, see exact P-value in Supplementary Data 17). e The heatmap on the right panel shows the transcripts abundance of each gene involved in the metabolic pathway of FFA (Five abundance categories 1–5 based on normalized count value, see Supplementary Information). Each condition has three biological replicates (R1, R2, R3). The significant difference (absolute log2[fold change] value > 1 and FDR adjusted P < 0.05) is indicated with an asterisk (see Supplementary Data 16). The pink and mediumaquamarine asterisks denote the significant increase and decrease of gene transcript abundance, respectively. The numerals behind the asterisks show the binned transcript abundance for four kinds of different conditions. The left panel shows the metabolic pathway of FFA biosynthesis and the desaturation pathway of C16- and C18- FFA. The triangles in pink, mediumaquamarine, and gray indicate significant increase, decrease, and no change of gene transcription, respectively. The circles in pink, mediu-maquamarine, and gray indicate significantly increased, decreased, and unchanged metabolites. For proteins encoded by multiple copies of genes, the changes in total transcripts of the genes were employed for determining the overall change according to gene transcript abundance (see details in Supplementary Information). Abbreviations are explained in Supplementary Data 18.