Fig. 6: Analysis of sialoglycan distribution in mouse intestine by tetra-GRAB staining.
From: Mutant glycosidases for labeling sialoglycans with high specificity and affinity
a Mouse large intestine sections were incubated with AF488-labeled anti-EpCAM antibody, tetra-GRAB-Sia-AF647, and tetra-GRAB-Sia3-AF555, followed by fluorescence microscopy analysis. Scale bars, 500 μm and 50 μm for zoomed-out and zoomed-in images, respectively. Representative images were shown from three independent biological replicates. b Schematic showing the spatial distribution of total and α2,3-linked sialoglycans in the mouse intestine revealed by tetra-GRAB staining. Created in BioRender. Liang, S. (2025) https://BioRender.com/g78q224. c Heatmap showing the scaled expression of genes involved in sialoglycan biosynthesis at the proximal and distal colon. The heatmap was generated using a public RNA-seq dataset49. Source data are provided as a Source Data file.
