Fig. 2: Tex14m is a hypomorphic allele of Tex14.
From: Intercellular bridges are essential for transposon repression and meiosis in the male germline
a Western blot analysis of TEX14 and VINCULIN (loading control) in testis extracts from 10-dpp animals. Relative TEX14 signal intensities normalized to VINCULIN are indicated below. Two mice were examined for each genotype. b TEX14 immunofluorescence on whole-mounted seminiferous tubules rendered in 3D. Higher magnification views of the regions indicated with white boxes are shown on the right. Two Tex14+/+ and three Tex14m/m mice were examined. c Immunofluorescence of TEX14 and MKLP1 on adult testis sections. Spermatocytes (Sc), elongating spermatids (eSt), and round spermatids (rSt) are indicated. TEX14 and MKLP1 staining is highlighted with yellow, dashed circles. Higher magnification views of TEX14 and MKLP1 colocalization are shown in insets. One Tex14m/m and two Tex14-/- mice along with control littermates were examined. d Electron micrograph of intercellular bridges in testis sections. Cell boundaries are depicted with red, dashed lines, intercellular bridges are indicated with yellow, dashed boxes. A higher magnification view of intercellular bridges is shown on the right and yellow arrows point to the electron-dense lining of intercellular bridges. Two mice were examined for each genotype. Source data are provided as a Source Data file.
