Fig. 4: Tex14 is required for meiotic DNA replication.
From: Intercellular bridges are essential for transposon repression and meiosis in the male germline
a STRA8 and EdU immunostaining on whole-mounted seminiferous tubules. Zoomed-in views of the regions indicated with yellow boxes are shown on the right and STRA8-positive EdU-negative cells are annotated (yellow arrows) and depicted on the far right. Two mice were examined for each genotype. b Immunofluorescence of STRA8 and EdU on testis sections. Higher magnification views of the regions indicated with white boxes are shown on the right. Three Tex14m/m and three Tex14-/- mice along with control littermates were examined. c Quantitation of the numbers of STRA8-positive EdU-negative (pink) and STRA8- EdU-double-positive (aqua) cells within tubule sections. Graphs represent individual mice and vertical bars represent individual tubules in a testis section. d Quantitation of STRA8- EdU-double-positive cells. The numbers of STRA8- EdU-double-positive cells counted in individual tubule sections that contain at least one STRA8-positive cell are shown in the dot plot above. Blue horizontal lines indicate means; bars indicate standard deviations. Proportions of tubules counted that contain varying ranges of double-positive cells are shown in the bar plot below. Mean values across mice are plotted; red bars indicate standard deviations. e Histograms of nuclei count as a function of the intensity of DAPI in STRA8-positive DMRT1-negative spermatocytes. Two mice were examined for each genotype. Source data are provided as a Source Data file.
