Fig. 6: Tex14m/m mutants exhibit transposon derepression in the male germline.
From: Intercellular bridges are essential for transposon repression and meiosis in the male germline
a SYCP3 and ORF1p immunofluorescence on testis sections. Tubules with numerous bright ORF1p-positive cells are indicated with white arrows. Three mice were examined for each genotype. b Distribution of ORF1p-positive tubules. c Tubules indicated in a with higher magnification views of boxed regions at the bottom. Cells were staged based on SYCP3-staining patterns: leptotene (L), zygotene (Z), pachytene (P), diplotene (D). d. Three Tex14m/m mice were examined. d ORF1p and VINCULIN (loading control) immunoblot in testis extracts from 13-week-old mice. Two Tex14m/m and three control littermates were examined. e L1Mda_l_5end RNA FISH with ORF1p immunostaining on testis sections. Higher magnification views of regions indicated with white arrowheads are shown below. Examples of colocalization of L1Mda_l_5end RNA and ORF1p are highlighted using white arrowheads. Two Tex14m/m mice were examined. Source data are provided as a Source Data file.
